Molecular diversity of nativeTrichodermaisolates againstFusarium oxysporumf. sp.lycopersici(Sacc.). A casual agent of Fusarium wilt in tomato (Lycopersicon esculentumMill.)

Autor:	K. Praveen Kumar, S. Shalini Devi, S. Saritha, Y. Sreenivasulu, M. Ram Kumar, P. Sudhakar
Rok vydání:	2012
Předmět:	biology food and beverages biology.organism_classification Lycopersicon Fusarium wilt RAPD Horticulture Fusarium oxysporum f.sp. lycopersici Trichoderma Fusarium oxysporum Botany Internal transcribed spacer Antagonism Agronomy and Crop Science
Zdroj:	Archives Of Phytopathology And Plant Protection. 45:686-698
ISSN:	1477-2906 0323-5408
DOI:	10.1080/03235408.2011.591195
Popis:	Fusarium wilt in tomato caused by Fusarium oxysporum is the one of the problematic diseases. In this study, 12 native Trichoderma isolates were isolated from different land use types in Rayalaseema region of Andhrapradesh, India and were tested for antagonistic activity against F. oxysporum using dual culture method; the maximum inhibition occurred in WT2 (78.4%) compared to the control. Molecular characterisation using random amplified polymorphic DNA (RAPD) technique reported 91.8% polymorphism among 12 isolates of Trichoderma. Internal transcribed spacer (ITS) region of rDNA amplification with genus-specific ITS1 and ITS4 universal primers produced amplicon size from 569 bp in all the isolates. The study resulted in identification of good competitive Trichoderma isolates against F. oxysporum. A relationship was found between the polymorphism showed by the Trichoderma isolates and their hardness to F. oxysporum during antagonism. Also, exhibition of sufficient genetic polymorphism aids further exploitat...
Databáze:	OpenAIRE
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