| Autor: |
Vânia Marilande Ceccatto, Juliana Osório Alves, Sávio Victor Diógenes Mendes, Paulo Elesson Guimarães de Oliveira, Raquel Martins de Freitas, Jonathan Elias Rodrigues Martins, Christina Pacheco, Stela Mirla da Silva Felipe |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of Applied Life Sciences International. :10-16 |
| ISSN: |
2394-1103 |
| Popis: |
Aims: The present study aimed to stablish and characterize an optimized protocol conformation to obtain adequate RNA quality from rodents skeletal muscle samples for sequencing studies. Place and Duration of Study: The in vivo experiments and analyses were performed in the Laboratory of Biochemistry and Gene Expression – LABIEX of the Superior Institute of Biomedical Science – ISCB from the State University of Ceará - UECE. Between 2017-2020. Methodology: Were used 23 samples from male Wistar rat skeletal muscle, specifically from soleus muscle. Total RNA extraction was performed using the classic TRIzol® method and commercial kit, merging steps from both. Capillary electrophoresis in the Bioanalyzer platform was used for RNA quality evaluation. Results: (C) Analyzes of adapted protocol RNA concentration, RIN and rate 28S/18S showed satisfactory results. 28S/18S Ribosomal bands appear well defined, without small traces, which indicates RNA with high integrity and without contamination of genomic DNA. Conclusion: Obtained RNA quality and integrity data satisfied the exigencies for posterior RNA-seq. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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