Equine Cardiodilatin/ Atrial Natriuretic Peptide
| Autor: | H. Mifune, Rudolf Richter, H. J. Mägert, W.-G. Forssmann, P. Schulz-Knappe |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Histology Immunoelectron microscopy Prohormone Biology Peptide hormone NPR1 NPR2 Natriuresis Endocrinology Atrial natriuretic peptide Internal medicine cardiovascular system medicine Anatomy Peptide sequence hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Cells Tissues Organs. 162:185-193 |
| ISSN: | 1422-6421 1422-6405 |
| DOI: | 10.1159/000046433 |
| Popis: | Cardiodilatin (CDD)/atrial natriuretic peptide (ANP) is a 28-amino acid peptide hormone known to be synthesized in the heart of a large number of different vertebrates. It plays an important role in the regulation of blood pressure and natriuresis/diuresis. Since the cardiovascular system of the horse has to meet the highest requirements concerning its physiological performance, we intended to characterize the cardiodilatin/atrial natriuretic peptide system of this species. By means of immunohistochemistry and immunoelectron microscopy, we precisely identified auricular cardiocytes as the loci of CDD/ANP synthesis. Using aortic smooth muscle relaxation assay and CDD/ANP-ELISA, we succeeded in isolating the biologically active prohormone. We subsequently cloned the equine cDNA of the CDD/ANP precursor protein and deduced its primary sequence. The entire precursor protein is in good agreement with the CDD/ANP prohormones of other mammals. The deduced theoretical average Mr of equine CDD/ANP-1-126 is 13,764, corresponding to the molecular weight of purified peptide determined by ESI-MS. Our findings suggest that equine CDD/ANP is produced in auricular cardiocytes and the predominant storage form of CDD/ANP in the auricle is the prohormone CDD/ANP-1-126. |
| Databáze: | OpenAIRE |
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