Non-linear photoacoustic and fluorescence microscopy using a modulated laser diode
| Autor: | T. Berer, G. Langer |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Photoacoustic effect
Materials science Laser diode business.industry 02 engineering and technology 021001 nanoscience & nanotechnology Laser 01 natural sciences law.invention Semiconductor laser theory 010309 optics Photoacoustic Doppler effect Wavelength Optics law 0103 physical sciences Microscopy Continuous wave Optoelectronics 0210 nano-technology business |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.2213027 |
| Popis: | We present simultaneous photoacoustic and fluorescence microscopy using a modulated laser diode. Photoacoustic waves and modulated fluorescence are generated by using a laser diode with a wavelength of 405nm at modulation frequencies in the Megahertz range. Additionally, a continuous wave offset radiation can be superposed. The excitation light is focused to the sample using a high NA objective. Luminescence is collected by the same objective in a confocal configuration and measured by an avalanche photo diode. Photoacoustic waves are recorded on the opposite site of the sample using a hydrophone. Acoustic and luminescence signals are recorded using a lock-in technique. We present photoacoustic imaging on red blood cells and multimodal imaging on fluorescent microspheres. Nonlinear photoacoustic and luminescence effects were introduced with the help of a superposed continuous wave laser. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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