Influence of Epidermal Growth Factor with Cysteamine on in -Vitro Buffalo Embryo Development
| Autor: | Al-shimaa Al-H. H. El-Naby, Youssef Youssef, Gamal A. Sosa, Tarek Scholkamy, Mahmoud Abou El-Roos, Karima Ghoneimy M. |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
030219 obstetrics & reproductive medicine
Antioxidant business.industry medicine.medical_treatment Embryogenesis 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences Cleavage (embryo) 040201 dairy & animal science In vitro Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure chemistry Epidermal growth factor embryonic structures medicine Cysteamine Blastocyst business |
| Zdroj: | Egyptian Journal of Veterinary Sciences. 47:27-39 |
| ISSN: | 2357-089X |
| DOI: | 10.21608/ejvs.2016.1080 |
| Popis: | FOR IMPROVING embryo development in buffalo, two experiments were conducted. The first one was carried out to evaluate the different concentrations (0, 5, 25, 50 ng/ml) of epidermal growth factors (EGF) on developmental competence of buffalo oocytes. The selected oocytes were cultured in the four concentrations of EGF. The embryo cleavage rate was significantly higher in oocytes exposed to 5, 25, 50 ng/ml EGF than control. There were no significant difference in four groups (0, 5, 25, 50 ng/ml EGF) in the rate of morula. But, better cleavage and blastocyst rates were observed at 5 ng/ml EGF. In the second experiment, the additive effect of 5 ng/ml EGF with 50 µM cysteamine on maturation and embryo development was studied. Oocytes were collected, matured and cultured in three groups. In the first group, media supplemented with 5 ng/ml EGF + 50 µM cysteamine combination. In the second group, media supplemented with EGF. The third group was supplemented with cysteamine. There was a significant increase in cleavage rate in combination group than EGF (P< 0.05) and cysteamine (P< 0.01) groups. But there was no significant difference in cleavage rate between EGF and cysteamine. The morula percentage was nearly similar in the three groups. But blastocyst rate was significantly (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|