| Autor: |
Max Heck, H. Brunings, A. M. C. Bergmans, J. F. P. Schellekens, W. K. van der Zwaluw, A. van der Zee, M. F. Peeters, W J B Wannet, C. E. Elzenaar, E. Thijssen, Henny M.E. Maas |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Legionella |
| DOI: |
10.1128/9781555817985.ch50 |
| Popis: |
When detailed epidemiologic characterization is required, standardized molecular typing methods are indispensable for the subtyping of Legionella pneumophila serogroups. Therefore, a comparison was made between pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) analysis for genotyping of L. pneumophila, using standardized protocols from the European Working Group on Legionella Infections (EWGLI). DNA fingerprint patterns derived from AFLP seem to be superior to the patterns derived from PFGE, in both speed and interpretability. AFLP and PFGE have similar discriminatory power. AFLP and PFGE are powerful tools for the epidemiological subtyping of L. pneumophila. The authors emphasize the importance of the standardization of data analysis and data exchange using the latest software (BioNumerics). Studies are under way to determine both the intralaboratory and interlaboratory reproducibility more extensively for further standardization of these powerful subtyping methods used by an increasing number of European countries. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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