HLA-A, HLA-B, and HLA-DRB1 Allele and Haplotype Frequencies in Renal Transplant Candidates in a Population in Southern Brazil
| Autor: | Erika Noda Noguti, Sueli Donizete Borelli, Roger Haruki Yamakawa, Gustavo Borelli Bedendo, Sérgio Seiji Yamada, Waldir Veríssimo da Silva Junior, Patricia Keiko Saito |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Genetics education.field_of_study Biochemistry (medical) Clinical Biochemistry Haplotype Population Public Health Environmental and Occupational Health Hematology Human leukocyte antigen Biology HLA-B HLA-A 03 medical and health sciences Medical Laboratory Technology 030104 developmental biology 0302 clinical medicine Immunology Immunology and Allergy Allele education Allele frequency HLA-DRB1 030215 immunology |
| Zdroj: | Journal of Clinical Laboratory Analysis. 30:258-265 |
| ISSN: | 0887-8013 |
| DOI: | 10.1002/jcla.21846 |
| Popis: | Background Very few studies have examined the diversity of human leukocyte antigens (HLA) in the Brazilian renal transplant candidates. Methods The frequencies of the HLA-A, HLA-B, and HLA-DRB1 alleles, haplotypes and phenotypes were studied in 522 patients with chronic renal failure, renal transplant candidates, registered at the Transplant Centers in north/northwestern Parana State, southern Brazil. Patients were classified according to the ethnic group (319 whites [Caucasians], 134 mestizos [mixed race descendants of Europeans, Africans, and Amerindians; browns or “pardos”] and 69 blacks). The HLA typing was performed by the polymerase chain reaction sequence-specific oligonucleotide method (PCR-SSO), combined with Luminex technology. Results In the analysis of the total samples, 20 HLA-A, 32 HLA-B, and 13 HLA-DRB1 allele groups were identified. The most frequent allele groups for each HLA locus were HLA-A*02 (25.4%), HLA-B*44 (10.9%), and HLA-DRB1*13 (13.9%). The most frequent haplotypes were HLA-A*01-B*08-DRB1*03 (2.3%), A*02-B*44-DRB1*07 (1.2%), and A*03-B*07-DRB1*11 (1.0%). Significant differences (P < 0.05) were observed in the HLA-A*68, B*08, and B*58 allele frequencies among ethnic groups. Conclusions This study provides the first data on the HLA-A, HLA-B, and HLA-DRB1 allele, phenotype and haplotype frequencies of renal transplant candidates in a population in southern Brazil. |
| Databáze: | OpenAIRE |
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