Characterization of Plasma membrane of winter wheat (TritiCum aestivum L.) Seedlings and Changes in ATP hydrolysing activity under low temparature stress

Autor:	N. V. Alyoshina, T. I. Trunova, K. K. Baruah, N. B. Astakhova
Rok vydání:	1990
Předmět:	chemistry.chemical_classification Chromatography biology Chemistry ATPase macromolecular substances Plant Science Horticulture Acclimatization chemistry.chemical_compound Membrane Enzyme ATP hydrolysis biology.protein Cold acclimation Azide Cold hardening
Zdroj:	Biologia Plantarum. 32:211-217
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02890876
Popis:	Plasma membranes were isolated from young seedlings of cold hardened and non-hardened winter wheat using aqueous polymer two phase system consisting of dextran-polyethyleneglycol. Electron microscopic analysis and activities of various marker enzymes revealed that the upper phase was enriched in plasma membrane and free from contamination by other membranes. The fraction thus obtained possessed Mg ATP hydrolysing activity with an optimum pH 6.5 and the activity was stimulated by K+. The activity was highly sensitive to orthovanadate, diethylstilbesterol, dicyclohexylcarbodimide, insensitive to azide and nitrate. It was suggested that the ATP hydrolysis was catalysed by H+ ATPase. Mg ATP hydrolysing activity in cold hardened seedlings was 38% higher than that from non-hardened seedlings.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4ea4a3422d3db358d57b8b162547892b https://doi.org/10.1007/bf02890876 Zobrazit plný text záznamu Full text from SpringerLink