Rapid detection of mycobacterial contamination in batches of whole cells of purifiedMycobacterium lepraeby pyrolysis gas chromatography

Autor:	G. Wieten, Françoise Portaels, D.G. Groothuis, J.J. Boon, David E. Minnikin
Rok vydání:	1984
Předmět:	Bacilli Chromatography biology Genetics Bacterial contaminants Gas chromatography Contamination biology.organism_classification Molecular Biology Microbiology Mycobacterium leprae Rapid detection Pyrolysis
Zdroj:	FEMS Microbiology Letters. 25:289-293
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1984.tb01474.x
Popis:	Preparations of Mycobacterium leprae, purified from host animal tissues, were screened by pyrolysis high-resolution gas-chromatographic analysis (Py-GC) for the presence of (myco)bacterial contaminants, which produce wax-ester mycolates. Contamination levels as low as 5% of the total number of acid-fast bacilli could be detected, based on the presence or absence of the marker compounds: a series of isomeric alkenes. The on-line pyrolysis capillary GC method provides an easy procedure for rapid quality control of preparations of purified M. leprae.
Databáze:	OpenAIRE
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