| Autor: | D. Dougal Burns, Christopher N. Jones, Susan J. Gregory, Michelle L. Teear, Jane F. Wright, Scott Jones, Denise Y. Lee |
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| Rok vydání: | 2001 |
| Předmět: |
Lanthanide
Sociology and Political Science Chemistry Clinical Biochemistry Fluorescence in the life sciences Photochemistry Biochemistry Acceptor Fluorescence Clinical Psychology Förster resonance energy transfer Fluorescence cross-correlation spectroscopy Time-resolved spectroscopy Time-resolved fluorescence energy transfer Law Spectroscopy Social Sciences (miscellaneous) |
| Zdroj: | Journal of Fluorescence. 11:13-21 |
| ISSN: | 1053-0509 |
| DOI: | 10.1023/a:1016691414432 |
| Popis: | The effect on fluorescence resonance energy transfer (FRET) of multiple labelling of DNA oligonucleotides with donor lanthanide chelate and acceptor CyDye™ fluors has been investigated. It is shown that using a multiple donor lanthanide chelate with a single acceptor Cy™ or Cy5 can increase sensitivity and fluorescence output. The enhanced FRET observed in the multiple donor label system has been utilised in two different DNA based assay formats to demonstrate the advantages over a steady state fluorescence assay and a radiometric assay. |
| Databáze: | OpenAIRE |
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