| Autor: |
Yasukazu Hozumi, Kaoru Goto, Zhonggang Feng, Toru Ichita, Tadashi Kosawada, Ken-ichi Konno |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
IFMBE Proceedings ISBN: 9783642293047 |
| DOI: |
10.1007/978-3-642-29305-4_74 |
| Popis: |
Although most cells are facing to mechanical stimuli, environment of cultured cells in an ordinary CO2 incubator may be inappropriate, because these cultured cells are normally free from mechanical stimuli. In this study, a novel three-dimensional micro vibration stage is developed to stimulate cultured cells mechanically. The simple two times twisted v-shaped steric structure of the stage makes possible to excite a culture dish three-dimensionally. By using the stage, the effect of vibration stimulation to adhesive cells was morphologically investigated. The experimental result using normal human osteoblast shows that the vibration stimulation decreases the projected area and increases the slenderness ratio of the cells. It suggests that the dynamic stimulation inhibits the morphological change according to progression of cellular senescence. A newly defined parameter, anti-aging effect, is proposed to describe the effect of the dynamic stimulation. It is found that the most effective vibration direction and frequency is unidirectional, horizontal and 10 Hz. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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