Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea)
| Autor: | Mikuláš Oros, František Marec, Alžbeta Radačovská, Zuzana Čadková, Irena Provazníková, Anna Marková, Martina Orosová |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Whole genome sequencing
Chromosome 7 (human) education.field_of_study Perch medicine.diagnostic_test Population Zoology Karyotype Parthenogenesis Biology biology.organism_classification Telomere Infectious Diseases medicine Animal Science and Zoology Parasitology education Fluorescence in situ hybridization |
| Zdroj: | Parasitology. 148:787-797 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182021000408 |
| Popis: | The large-sized tapeworm Dibothriocephalus latus is known as the broad or fish-borne cestode of mammals that is capable to infect humans and cause diphyllobothriosis. Recently, molecular data on D. latus has been accumulating in the literature and a complete genome sequence has been published; however, little is known about the karyotype and chromosome architecture. In this study, an in-depth karyological analysis of 2 D. latus specimens was carried out. The plerocercoids originated from a perch caught in subalpine Lake Iseo (Italy) and the tapeworms were reared in hamsters. Both specimens contained cells with a highly variable number of chromosomes ranging from18 to 27. Nevertheless, the largest portion of mitotic figures (47%) showed a number corresponding to the triploid set, 3n = 27. Accordingly, the karyotype of the analyzed specimens consisted of 9 triplets of metacentric chromosomes. Fluorescence in situ hybridization (FISH) with the 18S rDNA probe clearly demonstrated the presence of 3 clusters of hybridization signals on the triplet of chromosome 7, thus confirming the triploid status of the specimens. FISH with a telomeric (TTAGGG)n probe confined hybridization signals exclusively to the terminal chromosomal regions, supporting the earlier findings that this repetitive motif is a conserved feature of tapeworm telomeres. |
| Databáze: | OpenAIRE |
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