| Popis: |
Here we describedPerturbSci-Kinetics, a novel combinatorial indexing method for capturing three-layer single-cell readout (i.e.,whole transcriptomes, nascent transcriptomes, sgRNA identities) across hundreds of genetic perturbations. ThroughPerturbSci-Kineticsprofiling of pooled CRISPR screens targeting a variety of biological processes, we were able to decipher the complexity of RNA regulations at multiple levels (e.g.,synthesis, processing, degradation), and revealed key regulators involved in miRNA and mitochondrial RNA processing pathways. Our technique opens the possibility of systematically decoding the genome-wide regulatory network underlying RNA temporal dynamics at scale and cost-effectively. |
