Flavonoids: Efficient protectors of glucose-6-phosphate dehydrogenase from ultrasonic cavitation-induced inactivation

Autor:	D. I. Metelitza, V. P. Kurchenko, E. I. Karasyova
Rok vydání:	2007
Předmět:	chemistry.chemical_classification Flavonoid Morin Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Hesperidin Reaction rate constant chemistry Organic chemistry Glucose-6-phosphate dehydrogenase Astragalin Quercetin Naringin Nuclear chemistry
Zdroj:	Applied Biochemistry and Microbiology. 43:139-149
ISSN:	1608-3024 0003-6838
Popis:	Seven structurally diverse flavonoids have been shown to decrease glucose-6-phosphate dehydrogenase (G6PDH) inactivation in 0.1 M phosphate buffer (pH 7.4), induced by exposure to a high temperature (44°C), or by a low-frequency ultrasound (27 kHz, 60 Wt/cm2). The activity of the compounds was assessed by their ability to change effective first-order rate constants characterizing the total (thermal and ultrasonic), thermal, and ultrasonic inactivation of 2.5 nM G6PDH (k in, k*in, and k in(us), respectively). The value dependences of these constants on flavonoid concentrations (0.01–50 μM) were obtained. Rank order of potency exhibited by the compounds in protecting G6PDH appeared as follows: hesperidin > morin > silibin > naringin = quercetin > kampferol ≫ astragalin. The data obtained confirm the crucial role of free radicals formed in the field of ultrasonic cavitation (HO· and O 2 ·− in G6PDH inactivation in solutions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4e6f56821e21377050c2ddd8b231a3dc https://doi.org/10.1134/s0003683807020020 Zobrazit plný text záznamu Plný text ve formátu PDF