Molecular Analysis of the B Microchromosome in Steindachnerina insculpta (Characiformes: Curimatidae) by Microdissection

Autor:	Renata da Rosa, Ana Lúcia Dias, Tatiane Ramos Sampaio, Juceli Gonzalez Gouveia, Carlos Roberto Maximiano da Silva
Rok vydání:	2015
Předmět:	Genetics B chromosome biology Curimatidae Karyotype Retrotransposon Characiformes biology.organism_classification Homology (biology) Microchromosome Molecular Biology Genetics (clinical) Silurana
Zdroj:	Cytogenetic and Genome Research. 146:51-57
ISSN:	1424-859X 1424-8581
Popis:	B chromosomes are additional elements to standard karyotypes observed in different species of fishes, especially in Curimatidae. However, despite studies demonstrating the occurrence of Bs, little is known about their origin and evolution. To better understand the genomic composition and evolutionary processes involving B chromosomes, microdissection of B microchromosomes in Steindachnerina insculpta was conducted. Chromosome painting revealed the totally hybridized B and markings on A chromosomes both in S. in sculpta and in Cyphocharax spilotus, demonstrating a strong homology between these different species. In specimens of C. modestus, which do not have Bs, the signals were observed on A chromosomes. Cloning and sequencing of some B fragments revealed that the B microchromosome in S. insculpta is composed of repetitive elements, homologous to the DIRS-4 LTR retrotransposon of Xenopus (Silurana) tropicalis. FISH with clone pSi48 with the DIRS-4 retroelement revealed signals on all A chromosomes in the 2 species and also on the B, suggesting the insertion of repetitive elements in these species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4e6d4ab265fe0334c00ab226dd985114 https://doi.org/10.1159/000381932 Zobrazit plný text záznamu