| Autor: |
Myriana Vankova, Gila Behzadi, Michel Arluison |
| Rok vydání: |
1990 |
| Předmět: |
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| DOI: |
10.1016/s0074-7696(08)60669-5 |
| Popis: |
Publisher Summary This chapter outlines the advantages and drawbacks of contemporary methods of neuroanatomy when combined with immunocytochemical methods for the identification of the content of neuronal pathways. The particulate tracers that are currently used are wheat germ agglutinin (WGA) adsorbed to colloidal gold and fluorescent latex microspheres. The fact that these labels have been used in parallel and concurrently with wheat germ agglutinin–horseradish peroxidase (WGA–HRP) to study the afferent connections of various brain nuclei has allowed us to appreciate their sensitivity as retrograde tracers and also their ease of use in combination with immunocytochemistry. The regions of the rat brain that have been studied more are the bed nucleus stria terminalis (BNST), the ventral pallidum/subpallidal substantia innominata (VP/ SPSI), and the central nucleus of the amygdala (Ace). The increasing interest brought to the complex formed by these three nuclei lies principally in the fact that they may constitute a single anatomical-functional entity that links the motor and the limbic systems. The neuropeptide-containing pathways have been demonstrated directly by immunohistochemistry in young animals. The techniques that are introduced to analyze these pathways are summarized in two categories––namely, experimental lesions of the central nervous system (CNS) combined with biochemical or cytochemical methods and neuroanatomical methods combined with immunohistochemistry. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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