In vitro evaluation of anticancer and biological activities of synthesized manganese oxide nanoparticles
| Autor: | Maha Abdulla, Abdul Arif Khan, Shahanavaj Khan, Anees A. Ansari, Omar A Al Obeed, Rehan Ahmad |
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| Rok vydání: | 2016 |
| Předmět: |
Pharmacology
Programmed cell death Chemistry Organic Chemistry Pharmaceutical Science Biological activity 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Haemolysis 01 natural sciences Biochemistry In vitro 0104 chemical sciences Cell culture Apoptosis Drug Discovery Cancer research Molecular Medicine 0210 nano-technology Antibacterial activity Cytotoxicity |
| Zdroj: | MedChemComm. 7:1647-1653 |
| ISSN: | 2040-2511 2040-2503 |
| Popis: | This paper presents the results from a systematic study into the characterization and anticancer and biological activity of synthesized super-paramagnetic manganese oxide nanoparticles (Mn3O4-NPs). The Mn3O4-NPs showed an IC50 value of 666.44 μg mL−1 on the HT29 human colorectal cell line. Furthermore, we investigated the molecular mechanism of Mn3O4-NPs for the inhibition of Bcl-2 and Bcl-xL through an in vitro study. Bcl-2 and Bcl-xL are key signaling regulators of the programmed cell death pathway and ensure proper apoptosis and have been proposed as a novel target for anticancer therapy. The down-regulation of Bcl-2 and Bcl-xL on the HT29 cancer cell line showed that the NPs under study could be useful in the treatment of cancer. The down-regulation of the anti-apoptotic regulators Bcl-2 and Bcl-xL enhances cytotoxicity in colon carcinoma cells connected with the induction of apoptosis. Moreover, the in vitro haemolysis study antibacterial tests showed that Mn3O4-NPs have no significant toxicity and antibacterial activity, thereby exhibiting their biocompatibility and reliability in biomedical sciences. |
| Databáze: | OpenAIRE |
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