Immuno Expression of PAKs in Actinic Cheilitis and in Lip Squamous Cell Carcinoma
| Autor: | Simone de Sales Costa Moreira Carboni, Nayara Rubio Diniz Del Nero, Nanci Mendes Pinheiro, Ana Silva, Leopoldo Henrique Barboza Martins, Virgínia Oliveira Crema |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Kinase Cell growth Actinic cheilitis macromolecular substances Biology medicine.disease environment and public health Epithelium enzymes and coenzymes (carbohydrates) medicine.anatomical_structure medicine Immunohistochemistry biological phenomena cell phenomena and immunity Stratum spinosum Immunostaining Lip Squamous Cell Carcinoma |
| Zdroj: | Journal of Cytology & Histology. |
| ISSN: | 2157-7099 |
| DOI: | 10.4172/2157-7099.1000330 |
| Popis: | Lip squamous cell carcinoma (LSCC) may develop from a potentially malignant disorder known as Actinic cheilitis (AC). The p21-activated kinases (PAKs) are possible targets for cancer therapeutics. The LSSC and AC samples were analyzed by immunohistochemistry in order to detects endogenous levels PAKs group I (PAK1 and PAK2) and group II (PAK4, PAK5 and PAK6) only when they were in phosphorylated form (active). Keratinocytes of the basals stratum showed intense staining for phosphoPAKs 1/2, while the most superficial cells of the stratum spinosum and the surface layer showed moderate immunostaining for PAKs 4/5/6 phosphorylated in the epithelium adjacent to injured area. AC did not immunoexpress actives PAKs 1/2 and PAKs 4/5/6. LSSC did not immunoexpress phosphoPAKs 1/2, but some degenerating cells and cells in the necrotic area showed intense staining. Our results suggest that PAKs 1/2 not participate in the regulation of AC and LSCC pathogenesis, while PAKs 4/5/6 are involved in the regulation of cell death in LSCC. |
| Databáze: | OpenAIRE |
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