| Autor: |
Belinda B. Beezley, George K. Lewis, Nancy H. Ruddle, Joel W. Goodman |
| Rok vydání: |
1980 |
| Předmět: |
|
| Zdroj: |
Molecular Immunology. 17:925-931 |
| ISSN: |
0161-5890 |
| DOI: |
10.1016/0161-5890(80)90041-3 |
| Popis: |
T cell hybrids which express specificity for azobenzenearsonate (ABA) have been developed and analysed. Somatic cell hybrids were prepared between BW 5147, a thioguanine resistant AKR mouse T lymphoma and antigen-plate enriched T cells from ABA-mouse gamma globulin immunized A/J mice. Cells which grew in selective medium expressed both parental forms of hypoxanthine guanine phosphoribosyltransferase, isocitrate dehydrogenase and Thy l. T cell hybrids formed rosettes with antigen conjugated sheep and mouse erythrocytes which were inhibited by pre-exposure of the hybrid cells to soluble ABA-human serum albumin but not ovalbumin. T cell hybrids, in contrast to ABA B cell hybrids which were also studied here, did not rosette with antigen coupled human erythrocytes. T cell hybrids which were subcloned on agar maintained their rosetting activity, even to the extent of its inhibition by soluble antigen. These studies set the foundation for future experiments on the molecular analysis of surface membranes and products of antigen-specific T cell hybrids. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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