Agrobacterium-mediated In-planta transformation of bread wheat (Triticum aestivum L.)
| Autor: | Kanika Kumar, Priyanka Singh |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Inoculation Agrobacterium fungi food and beverages Plant Science Genetically modified crops Agrobacterium tumefaciens Biology biology.organism_classification 01 natural sciences 03 medical and health sciences Tissue culture Transformation (genetics) Horticulture 030104 developmental biology Agronomy and Crop Science 010606 plant biology & botany Biotechnology Southern blot Transformation efficiency |
| Zdroj: | Journal of Plant Biochemistry and Biotechnology. 31:206-212 |
| ISSN: | 0974-1275 0971-7811 |
| DOI: | 10.1007/s13562-021-00669-x |
| Popis: | Agrobacterium-mediated in-planta transformation method allows efficient plant transformation without tissue culture. In the present study, a tissue culture independent protocol for floral transformation in bread wheat was developed. Three bread wheat genotypes namely, HD 2967, HD 3086 and Bobwhite were used for floral transformation. At pre-anthesis stage, clipped florets were sprayed with inoculation medium containing Agrobacterium tumefaciens strain EHA 105 culture harboring binary vector pCAMBIA1301-p35S:hptII-p35S:gus and allowed to grow till maturity. Positive transformants were confirmed through antibiotic selection, PCR analysis and southern blotting. The method led to generation of transformation events in Bobwhite and HD 2967 wheat genotypes. The transformation efficiency of T1 transgenic plants was higher in Bobwhite as compared to HD 2967. Floral spray method of transformation developed in the present study is efficient for development of transgenic wheat and can be used for improvement of wheat using transgenic approach. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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