Mutations in HLA DRB1*0101 Exon 2 in a Sub-population of Latvia

Autor: Diana Kasjko, A. Sochnev, E. Eglite, Vladislavs Jasinskis, E. Dobele, Lilija Kovalchuka, Ludmila Viksna, Gunta Sture
Rok vydání: 2014
Předmět:
Zdroj: British Journal of Medicine and Medical Research. 4:981-989
ISSN: 2231-0614
Popis: Aims: To find out whether ongoing missense mutations in the exon 2 of DRB1*01:01 affect the operation of this protective allele in HIV patients. Place and Duration of Study: the Clinical Immunology and Immunogenetics Laboratory of Riga Stradiņs University, Riga Eastern Clinical University Hospital, “Infectology Center of Latvia”, between May 2006 and December 2011. Methodology: The study includes 200 HIV-infected patients. DNA was isolated from venous blood samples using the Qiagen QIAamp DNA kit reagents and the exon 2 nucleotide sequence of HLA was determined by the automatic sequencing – “Big Dye Terminator mix” (Applied Biosystems, USA). Statistical analysis was performed using Microsoft Excel, DOS StatCalc programs. The significance of the differences in Case Study British Journal of Medicine & Medical Research, 4(4): 981-989, 2014 982 indicators was evaluated according to reliability p≤0.05. The odds ratio was calculated according to Wolf’s method. Results: We found missense: at codon 47– in 80% of cases; at codon 67– in 20% of cases; at codon 75 – in 11% of cases; at codon 82– in 10% of cases; at codon 86– in 10% of cases (p
Databáze: OpenAIRE