Intracellular K+ and cyclic diphosphoglycerate pools and transients in Methanobacterium thermoautotrophicum
| Autor: | Robert J. Seely, David E. Fahrney, Ray D. Krueger |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Methanobacterium
biology Methanobacteriaceae Potassium chemistry.chemical_element Chemostat Metabolism Phosphate biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound chemistry Biochemistry Extracellular Biophysics Diphosphoglycerate Ecology Evolution Behavior and Systematics |
| Zdroj: | Systematic and Applied Microbiology. 7:388-392 |
| ISSN: | 0723-2020 |
| DOI: | 10.1016/s0723-2020(86)80039-x |
| Popis: | Summary Cultivation of M. thermoautotrophicum under K + - and phosphate-limiting conditions in chemostat cultures provided evidence that this organism can grow rapidly without the need to maintain high internal concentrations of K + and cyclic diphosphoglycerate (cyclic DPG). The internal K + concentration during K + limitation was about 320 mM. For comparison, the K + concentration was near 800 mM in batch-grown cells containing about 150 mM cyclic DPG. Phosphate limitation resulted in a decline in the K + and cyclic DPG pools to 200 mM and 9 mM, respectively. Pulse addition of K + to a K + -limited chemostat culture resulted in a transient increase in cellular K + and cyclic DPG before the new growth rate was reached. A large increase in extracellular K + (from 0.02 to 1 mM) resulted in a transition to a phosphate-limited culture and a concomitant decrease in cell K + and cyclic DPG. These two components declined by approximately 100 mM and 30 mM, respectively, during the transition from K + to phosphate limitation. Pulse addition of phosphate to phosphate-limited chemostat cultures resulted in a transient expansion of the K + and cyclic DPG pools before the shiftup in growth rate occurred. These adjustments of K + to cyclic DPG levels established K + as the major cation balancing the −3 charge of cyclic DPG. |
| Databáze: | OpenAIRE |
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