| Popis: |
Serpin (Serine Protease Inhibitor) widely distributes in animals, plants, protozoan, prokaryotes and viruses. Serpin-6 belongs to the superfamily of serine protease inhibitor. In this study, we constructed a prokaryotic vector - pET28a-serpin-6, and used IPTG (isopropyl thiogalactoside) to induce serpin-6 protein expression, then applied the Ni-affinity chromatography to purify the collected recombinant protein. The assay of SDS-PAGE and Anti-his Polyclonal antibody Test showed that the recombinant serpin-6 protein have a molecular weight of 45 kDa. The highly purified sample of the recombinant protein was obtained. The protein has been used as antigen to immunizeKunMing miceusing the 4 times immunization method. We successfully attained the polyclonal antibody of serpin-6.The titer of the antibody is as high as 1:20000, with a good specificity. The polyclonal antibody can provide a practical tool to further study the distribution of protein expression and functions of serpin-6 in different states and breeds ofBombyx mori. |
