Flow cytometry beads rather than the antihuman globulin method should be used to detect HLA Class I IgG antibody (PRA) in cadaveric renal regraft candidates
| Autor: | Nicolas A. Muruve, Scott B. McDonald, Daniel Murillo, Alan M. Luger, Mark I. Aeder, Charles F. Shield, Paul W. Nelson, Karen A. Baier, Bradley A. Warady, Christopher F. Bryan |
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| Rok vydání: | 2002 |
| Předmět: |
Antihuman globulin
Transplantation animal structures medicine.diagnostic_test biology business.industry Panel reactive antibody Human leukocyte antigen urologic and male genital diseases Molecular biology female genital diseases and pregnancy complications Immunoglobulin G Flow cytometry Histocompatibility Coombs test Immunology medicine biology.protein Antibody business hormones hormone substitutes and hormone antagonists circulatory and respiratory physiology |
| Zdroj: | Clinical Transplantation. 16:15-23 |
| ISSN: | 0902-0063 |
| DOI: | 10.1034/j.1399-0012.16.s7.2.x |
| Popis: | HLA Class I antibody screening can be performed by flow cytometry using a mixture of 30 distinct bead populations each coated with the Class I antigen phenotype derived from different cell lines. In this study we compared the efficacy of Class I antibody screens done by flow cytometry beads with the antihuman globulin (AHG) method for patients awaiting cadaveric renal retransplantation. Class I panel reactive antibody (PRA) screening by flow cytometric beads of 21 regraft serum samples that had all been found to be negative by AHG DTT Class I PRA, revealed that 57.1% (12 of 21) had a flow Class I PRA of > or = 10%. Furthermore, when five regraft sera with an intermediate PRA were screened (mean AHG DTT PRA = 33.2 +/- 13%) the mean flow Class I PRA almost doubled (mean flow PRA = 72.4 +/- 10.2%) (p or = 80%), the incidence of a positive flow cross-match was 0%, 72% and 85% and the incidence of retransplantation was 60%, 22% and 10%, in each of these groups, respectively. These data provided our histocompatibility laboratory with the rationale to stop performing the AHG PRA and perform only the flow Class I PRA method for regraft candidates. |
| Databáze: | OpenAIRE |
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