Model of in vitro Healing to Test the Influence of Dedifferentiated Crithmum maritimum Cells on Dermal Repair and Epidermal Regeneration

Autor:	C. Lequeux, Odile Damour, C. Montastier, M.R. Rovere, A. Lhoste
Rok vydání:	2010
Předmět:	Pharmacology integumentary system biology Physiology Cellular differentiation Regeneration (biology) Totipotent Dermatology General Medicine biology.organism_classification In vitro Cell biology Crithmum Botany Skin equivalent Stem cell Wound healing
Zdroj:	Skin Pharmacology and Physiology. 24:75-80
ISSN:	1660-5535 1660-5527
Popis:	Background: The aim was to test the influence of dedifferentiated Crithmum maritimum cells (dCMC), totipotent vegetal stem cells, on epidermal regeneration in perfect homeostasis using a skin equivalent (SE) model. Materials and Methods: SE are prepared by seeding fibroblasts on a collagen-glycosaminoglycan-chitosan dermal substrate (DS) epidermalized by keratinocytes 3 weeks later. The originality of this present study lies in the systemic administration of dCMC from the moment when fibroblasts are seeded in the DS right through to the reconstruction of the SE. The thickness of the epidermis as well as the number of proliferating cells expressing Ki-67 and layers expressing terminal differentiation marker (filaggrin) were compared in the dCMC-treated SE versus an untreated control group. Results: dCMC accelerated the complete regeneration and differentiation of the epidermis compared to the negative control (35 days instead of 42 days). Histology showed a multilayered, thick and differentiated epithelium after 35 days of culture. The basal and suprabasal layers had increased 4.88 ± 0.41 times versus the negative control (Mann-Whitney U test: p < 0.001). This result was attributed to the greater proliferation of basal cells because the cell numbers expressing the Ki-67 proliferation marker had increased significantly compared to the negative control (Mann-Whitney U test: p < 0.001). Moreover, dCMC allowed the differentiated epithelium to recover because only treated SE expressed the terminal differentiation marker filaggrin. Conclusion: Our data show that dCMC enhance epidermal cell grafts by stimulating their regeneration and differentiation in perfect homeostasis. They allow the epidermis to recover its structure for protective functions faster than the negative control.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4dc7c80c1a67ade0c646ccfb2536cbcc https://doi.org/10.1159/000321991 Zobrazit plný text záznamu