Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation
| Autor: | Xian-jun Dai, Ming-qi Liu, Xin Xu, Rong-fa Guan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
biology
Bran Chemistry Process Chemistry and Technology Aspergillus niger General Chemistry equipment and supplies biology.organism_classification Xylan Catalysis Chitosan chemistry.chemical_compound Biochemistry Xylanase Magnetic nanoparticles human activities Xylooligosaccharide Nuclear chemistry Thermostability |
| Zdroj: | Catalysis Communications. 55:6-10 |
| ISSN: | 1566-7367 |
| DOI: | 10.1016/j.catcom.2014.06.002 |
| Popis: | Aspergillus niger xylanase A (XylA) was immobilized onto Fe3O4-coated chitosan magnetic nanoparticles prepared by the layer-by-layer self-assembly approach. The Fe3O4-coated chitosan magnetic nanoparticles showed a high binding capacity of 162.2 mg ∙ g− 1-particles and a recovery activity of 56.5% for XylA. The immobilized XylA showed improved thermostability and storage stability compared with free XylA. The immobilized XylA retained 87.5% activity after seven successive reactions by magnetic separation. Xylotriose and xylohexaose were the main products released from birchwood xylan and wheat bran insoluble xylan by immobilized XylA, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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