| Autor: |
Atousa Aliahmadi, Hasan Rafati, Maryam Kabiri, Alireza Ghassempour, Masoud Tohidfar, Hassan Rezadoost, Zeinab Shahsavari |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.21203/rs.3.rs-828077/v1 |
| Popis: |
Over-consumption of antibiotics has led to antimicrobial resistance creating urgency to find fast and efficient techniques to monitor the level of antibiotics in the foods. The advantages of using aptamer for a variety of scientific challenges are becoming more abundant in different fields, making its applications more effective and cost-efficient. In this study, two aptamers against two quinolones antibiotics (Enrofloxacin and Ofloxacin) were isolated via GO-SELEX (graphene oxide assisted Systematic Evolution of Ligands by Exponential Enrichment). The interaction between the aptamer and its cognate antibiotic was investigated using spectroscopy and mass spectrometry. The obtained evidence from spectroscopy showed that the interaction of antibiotics with their aptamers resulted in a 41.98% and 22.45 % reduction in the extinction coefficient (ɛ) value of enrofloxacin and ofloxacin, respectively. This phenomenon could arise from the stacking interaction between the aromatic region of antibiotic and aptamer’s bases. The formation of the aptamer-antibiotic complex was studied by the high-resolution ESI-Q-TOF. In the obtained mass spectra, several peaks corresponding to the aptamer-antibiotic complexes with a wide range of charge distribution were identified, which could be explained by the fact that both aptamer-ENR and aptamer-OFLO complexes were stable in the gas phase and the intact complexes survived in the vacuum. This stability could be explained by the hydrogen bonding and electrostatic interactions between the aptamers and antibiotics. As both aptamers can distinguish quinolone from other antibiotic families, these aptamers may become great recognizing elements for apta-sensors. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
