Constitutive Expression of Prostaglandin-Endoperoxide Synthase 2 by Somatic and Spermatogenic Cells Is Responsible for Prostaglandin E2 Production in the Adult Rat Testis1
| Autor: | Wendy R. Winnall, Mark P. Hedger, Penelope Alexandra Falshaw Whiley, Moira K O'Bryan, Ugur Ali, Jonathan J. Hirst, Julie A. Muir |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Cell type Somatic cell PTGS1 Cell Biology General Medicine In situ hybridization Biology Sertoli cell Molecular biology Blot Prostaglandin-endoperoxide synthase 2 chemistry.chemical_compound medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Internal medicine medicine lipids (amino acids peptides and proteins) Prostaglandin E2 medicine.drug |
| Zdroj: | Biology of Reproduction. 76:759-768 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.106.053124 |
| Popis: | Prostaglandins (PGs), particularly PGE2, have been implicated in the control of testicular steroidogenesis, spermatogenesis, and local immunity. However, virtually nothing is known about the expression or activity of the prostaglandin-endoperoxide synthases (PTGSs; also referred to as the cyclooxygenases), the specific rate-limiting enzymes responsible for PG production, in the adult testis. This activity was investigated in rats under normal conditions and during lipopolysaccharide-induced inflammation using quantitative real-time PCR, in situ hybridization, Western blotting, and PGE2 measurements by ELISA. The mRNA for both the “constitutive” Ptgs1 and the “inducible” Ptgs2 forms was detected in multiple testicular cell types. Testicular Ptgs2 expression was substantially higher than that of Ptgs1, and testicular production of PGE2 in vitro was found to be suppressed by a specific PTGS2 inhibitor (NS-398), but not by an inhibitor of PTGS1. Further investigation indicated that 1) PGE2 production... |
| Databáze: | OpenAIRE |
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