Phenolic Analyses of Haskap Berries (Lonicera caeruleaL.): Spectrophotometry Versus High Performance Liquid Chromatography

Autor: Rabie Khattab, Marianne Su-Ling Brooks, Amyl Ghanem
Rok vydání: 2016
Předmět:
Zdroj: International Journal of Food Properties. 19:1708-1725
ISSN: 1532-2386
1094-2912
DOI: 10.1080/10942912.2015.1084316
Popis: Haskap berries (Lonicera caerulea L.) are known for their high phenolics, anthocyanins, and antioxidant potential. The data on the phenolic profile of these fruits are lacking. In this study, the phenolic profiles of three haskap varieties; tundra, berry blue, and indigo gem grown in Nova Scotia, Canada were investigated for the first time using spectrophotometery and high-performance liquid chromatography. Berries were analyzed for total phenolic content/total reducing capacity, total anthocyanin content, and antioxidant potential (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity). The total reducing capacity, total anthocyanin content, and 2,2-diphenyl-1-picrylhydrazyl values were 6.17–8.42 mg gallic acid equivalents/gram fresh weight, 4.49–6.97 mg cyanidin-3-glucoside equivalents/gram fresh weight, and 78.70–89.55%, respectively. The extracts were analyzed by reversed-phase diode array detector–high-performance liquid chromatography through a gradient elution using Synergi 4 µm Max-RP C12 colu...
Databáze: OpenAIRE
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