Phenolic Analyses of Haskap Berries (Lonicera caeruleaL.): Spectrophotometry Versus High Performance Liquid Chromatography
Autor: | Rabie Khattab, Marianne Su-Ling Brooks, Amyl Ghanem |
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Rok vydání: | 2016 |
Předmět: |
Chromatography
medicine.diagnostic_test biology Chemistry Lonicera caerulea 04 agricultural and veterinary sciences Berry biology.organism_classification 040401 food science High-performance liquid chromatography Indigo chemistry.chemical_compound 0404 agricultural biotechnology Spectrophotometry Anthocyanin medicine Gallic acid Food Science Gram |
Zdroj: | International Journal of Food Properties. 19:1708-1725 |
ISSN: | 1532-2386 1094-2912 |
DOI: | 10.1080/10942912.2015.1084316 |
Popis: | Haskap berries (Lonicera caerulea L.) are known for their high phenolics, anthocyanins, and antioxidant potential. The data on the phenolic profile of these fruits are lacking. In this study, the phenolic profiles of three haskap varieties; tundra, berry blue, and indigo gem grown in Nova Scotia, Canada were investigated for the first time using spectrophotometery and high-performance liquid chromatography. Berries were analyzed for total phenolic content/total reducing capacity, total anthocyanin content, and antioxidant potential (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity). The total reducing capacity, total anthocyanin content, and 2,2-diphenyl-1-picrylhydrazyl values were 6.17–8.42 mg gallic acid equivalents/gram fresh weight, 4.49–6.97 mg cyanidin-3-glucoside equivalents/gram fresh weight, and 78.70–89.55%, respectively. The extracts were analyzed by reversed-phase diode array detector–high-performance liquid chromatography through a gradient elution using Synergi 4 µm Max-RP C12 colu... |
Databáze: | OpenAIRE |
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