Development of an enzyme-linked immunosorbent assay for the .beta.-exotoxin of Bacillus thuringiensis

Autor:	Anne D. Lucas, Bruce D. Hammock, Robert O. Harrison, Shirley J. Gee, Hassan K. M. Bekheit
Rok vydání:	1993
Předmět:	Antiserum Serum albumin Heterologous General Chemistry Biology medicine.disease_cause biology.organism_classification Molecular biology Antigen Bacillus thuringiensis medicine biology.protein bacteria Antibody General Agricultural and Biological Sciences Exotoxin Keyhole limpet hemocyanin
Zdroj:	Journal of Agricultural and Food Chemistry. 41:1530-1536
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf00033a033
Popis:	A competitive enzyme-linked immunosorbent assay (ELISA) was developed to quantify the amount of β-erotorin from Bacillus thuringiensis in solution and to evaluate the ability of the antibodies to distinguish among various natural and synthetic compounds related to the β-exotoxin. The β-exotoxin was coupled to several proteins via glutaraldehyde, diazotization, and periodate procedures. The antibodies used in the assay were obtained from antisera raised against β-exotoxin linked to keyhole limpet hemocyanin, and the ELISA coating antigens consisted of β-exotoxin-bovine serum albumin conjugates. Both homologous and heterologous ELISA systems were examined
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4d7e099ee8daaf733921f448138ef679 https://doi.org/10.1021/jf00033a033 Zobrazit plný text záznamu