HPLC determination of colistin and aminoglycoside antibiotics in feeds by post-column derivatization and fluorescence detection

Autor:	L. Németh-Konda, Gy. Morovján, P. P. Csokán
Rok vydání:	1998
Předmět:	Chromatography Organic Chemistry Clinical Biochemistry Aminoglycoside Kanamycin Neomycin Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Column chromatography chemistry Amikacin medicine Colistin Derivatization medicine.drug
Zdroj:	Chromatographia. 48:32-36
ISSN:	1612-1112 0009-5893
Popis:	Reversed-phase, HPLC methods employing post-column derivatization and fluorescence detection were developed for the determination of the peptide colistin and four aminoglycoside antibiotics in feeds. Extraction of the analytes was by sonication and shaking with dilute hydrochloric acid. Post-column derivatization was performed using orthophtaldialdehyde-2-mercaptoethanol chemistry. Assay of colistin was by using an acetonitrile-aqueous sodium sulphate-triethylammonium phosphate (pH 2.8) eluent. Aminoglycoside antibiotics:amikacin, kanamycin, gentamycin and neomycin were analyzed using a tetrahydrofuran-aqueous sodium sulphate-sodium pentanesulphonate-acetic acid mobile phase. The method was also applied to some pharmaceutical preparations. Preliminary results showed that the method can be adapted for the assay of the above antibiotics in meat and animal serum for residue and pharmacokinetic studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4d71dfaf2ecf9eb01561e9d8bd6992f6 https://doi.org/10.1007/bf02467512 Zobrazit plný text záznamu Full text from SpringerLink