Development of viscometric methods for studying the interaction of various porphyrins with DNA. Part I: meso-tetra-(4N-hydroxyethylpyridyl) porphyrin and its Ni-, Cu-, Co- and Zn- containing derivatives

Autor:	Vigen G. Barkhudaryan, Gayane V. Ananyan, Yeva B. Dalyan, Samvel G. Haroutiunian
Rok vydání:	2014
Předmět:	Relative viscosity Intercalation (chemistry) Cationic polymerization Viscometer General Chemistry DNA Solutions Porphyrin Metal chemistry.chemical_compound Crystallography chemistry visual_art visual_art.visual_art_medium Organic chemistry Absorption (chemistry)
Zdroj:	Journal of Porphyrins and Phthalocyanines. 18:594-599
ISSN:	1099-1409 1088-4246
DOI:	10.1142/s1088424614500357
Popis:	The influence of water soluble cationic meso-tetra(4N-hydroxyethyl)pyridyl porphyrin ( H 2 THOEtPyP 4) and it's metal complexes with Ni ( II ), Cu ( II ), Co ( II ) and Zn ( II ) on hydrodynamic and spectral behavior of DNA solutions has been studied by viscometry and UV-vis absorption methods. It was shown that the presence of planar porphyrins, such as H 2 THOEtPyP 4, NiTHOEtPyP 4 and CuTHOEtPyP 4 leads to an increase in viscosity at relatively small concentrations, and then decreases to stable values. Such behavior corresponds to intercalation of these porphyrins in DNA structure, which results in a decrease of helical twist and lengthening of the DNA molecule. In the case of porphyrins with axial ligands, such as ZnTHOEtPyP 4 and CoTHOEtPyP 4, the relative viscosity decreases, which is explained by self-stacking of these porphyrins on DNA surface. Calculation and interpretation of binding parameters (Kb and n) demonstrated good agreement of viscometric and spectrophotometric measurements.
Databáze:	OpenAIRE
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