Ru(bpy)32+-Silica@Poly-L-lysine-Au as labels for electrochemiluminescence lysozyme aptasensor based on 3D graphene
Autor: | Jian-Mei Cen, Heyou Han, Quanyou Chen, Yu-Qin Qiu, Min Liu, Defen Feng, Xiao Chen, Fangkai Du, Yeyu Wu, Xuecai Tan, Jun Yan, Hui Zhang, Shaogang Liu |
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Rok vydání: | 2018 |
Předmět: |
Detection limit
Graphene Chemistry Aptamer Biomedical Engineering Biophysics Substrate (chemistry) 02 engineering and technology General Medicine 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences law.invention chemistry.chemical_compound Colloidal gold law Electrode Electrochemistry Electrochemiluminescence Lysozyme 0210 nano-technology Biotechnology Nuclear chemistry |
Zdroj: | Biosensors and Bioelectronics. 106:50-56 |
ISSN: | 0956-5663 |
DOI: | 10.1016/j.bios.2018.01.059 |
Popis: | In this work, the feasibility of a novel sensitive electrochemiluminescence aptasensor for the detection of lysozyme using Ru(bpy)32+-Silica@Poly-L-lysine-Au (RuSiNPs@PLL-Au) nanocomposites labeling as an indicator was demonstrated. The substrate electrode of the aptasensor was prepared by depositing gold nanoparticles (AuNPs) on 3D graphene-modified electrode. The lysozyme binding aptamer (LBA) was attached to the 3D graphene/AuNPs electrode through gold-thiol affinity, hybridized with a complementary single-strand DNA (CDNA) of the lysozyme aptamer labeled by RuSiNPs@PLL-Au as an electrochemiluminescence intensity amplifier. Thanks to the synergistic amplification of the 3D graphene, the AuNPs and RuSiNPs@PLL-Au NPs linked to Ru(bpy)32+-ECL further enhanced the ECL intensity of the aptasensor. In presence of lysozyme, the CDNA segment of the self-assembled duplex was displaced by the lysozyme, resulting in decreased electrochemiluminescence signal. Under the optimized conditions, the decrease in electrochemiluminescence intensity varied proportionally with the logarithmic concentration of the lysozyme from 2.25 × 10-12 to 5.0 × 10-8 mol L-1, and the detection limit was estimated to 7.5 × 10-13 mol L-1. The aptasensor was further tested in real samples and found reliable for the detection of lysozyme, thus holding great potential application in food safety researches and bioassay analysis. |
Databáze: | OpenAIRE |
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