| Autor: |
J.N. Rabaud, Jean-Louis Goergen, G. Pierry, Jean-Marc Engasser, J. Hache, Annie Marc, I. Geahel, V. Geaugey |
| Rok vydání: |
1994 |
| Předmět: |
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| DOI: |
10.1016/b978-0-7506-1845-8.50069-7 |
| Popis: |
SUMMARY A new configuration of perfused reactor, with cells confined between two flat membranes, is presented. The flow rate of the medium forced through the membranes can be controled independently of the oxygen transport assured by a tubular membrane network. Results indicate negligible membrane fouling and culture duration up to 2 months without product retention. Cells protected against shear stress can be homogeneously irrigated and reached an estimated density of 6×107 cells/mL. Compared to the batch mode, the Cellys reactor allows a 3 times higher MAb concentration, a 2 times lower consumption of medium per gramme of MAb and a 100 times higher daily productivity per liter of cell chamber. Made out of medical grade materials, it is sterilizable and extrapolable. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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