| Autor: |
Maria Adelaide Pronzato, Damiano Cottalasso, Nicola Traverso, K H Cheeseman, Patrizio Odetti, Umberto M. Marinari |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Cell Biochemistry and Function. 14:149-154 |
| ISSN: |
0263-6484 |
| DOI: |
10.1002/cbf.658 |
| Popis: |
Oxidation and glycation are non-enzymatic protein modifications involved in the pathogenesis of aging. We evaluated their possible influences in an in vitro system: albumin was oxidized by gamma-irradiation and then exposed to glycation in vitro. Fluorescence modifications were analysed as signals of protein alterations. Both radiolytic oxidation and in vitro glycation provoked a sharp decrease of tryptophan fluorescence (278 nm ex./340 nm em.); their effects tended to be additive, unless a saturation limit was reached. Both individually and in combination, these two non-enzymatic processes induced the appearance of a new fluorescence (335 nm ex./415 nm em.); in this case as well there was an additive effect, with a trend toward saturation. Radiolytic oxidation and in vitro glycation seem to provoke similar damage to the exposed proteins: the observed fluorescence alterations may be due to similar conformational changes, breaks or the development of fluorophores. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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