Virulence status, viral accommodation and structural protein profiles of white spot syndrome virus isolates in farmedPenaeus monodonfrom the southeast coast of India
| Autor: | Shankar Vinayakarao Alavandi, Victor Stalinraj, Mohandas Sanjuktha, T.C. Santiago, Koyadan Kizhakedath Vijayan, C. P. Balasubramanian |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Aquaculture Research. 40:129-138 |
| ISSN: | 1365-2109 1355-557X |
| DOI: | 10.1111/j.1365-2109.2008.02071.x |
| Popis: | The objective of this study was to investigate the reason for variation in the virulence of white spot syndrome virus (WSSV) from diierent shrimp farms in the Southeast coast of India. Six isolates of WSSV from farms experiencing outbreaks (virulent WSSV; vWSSV) and three isolates of WSSV from farms that had infected shrimps but no outbreaks (non-virulent WSSV; nvWSSV) were collected from diierent farms in the Southeast coast of India. The sampled animals were all positive for WSSV by ¢rst-step PCR. The viral isolates were compared using histopathology, electron microscopy, SDS-PAGE analysis of viral structural proteins, an in vivo infectivity experiment and sequence comparison of major structural protein VP28; there were no diierences between isolates in these analyses. A signi¢cant observation was that the haemolymph protein pro¢le of nvWSSV-infected shrimps showed three extra polypeptide bands at 41, 33 and 24 kDa that were not found in the haemolymph protein pro¢le of vWSSV-infected shrimps. The data obtained in this study suggest that the observed diierence in the virulence of WSSV may not be due to any change in the virus, rather it could be due to the shrimp defence system producing certain factors that help it to accommodate the virus without causing any mortality. |
| Databáze: | OpenAIRE |
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