Nucleation and Growth of Membrane Protein Crystals In Meso—A Fluorescence Microscopy Study
| Autor: | Vadim Cherezov, Andrey Bogorodskiy, Georg Büldt, Vitaly Polovinkin, Alexey Mishin, Fedor Frolov, Valentin Gordeliy, Valentin Borshchevskiy, Thomas Gensch, Ekaterina Round |
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| Rok vydání: | 2015 |
| Předmět: |
Ostwald ripening
0303 health sciences Materials science biology Nucleation Crystal growth Bacteriorhodopsin General Chemistry 010402 general chemistry Condensed Matter Physics 01 natural sciences 0104 chemical sciences law.invention Crystal 03 medical and health sciences Crystallography symbols.namesake law Phase (matter) Fluorescence microscope symbols biology.protein General Materials Science Crystallization 030304 developmental biology |
| Zdroj: | Crystal Growth & Design. 15:5656-5660 |
| ISSN: | 1528-7505 1528-7483 |
| Popis: | Since the introduction of in meso crystallization of membrane proteins in lipidic cubic phase (LCP) by Landau and Rosenbusch in 1996, numerous studies attempted to elucidate the mechanism of in meso crystal nucleation and growth. Here we present a fluorescence microscopy study of the crystallization process of the light-driven proton pump bacteriorhodopsin. The crystallization starts with formation of microcrystals, followed by growth of a dominating crystal at the expense of smaller ones and formation of a depletion zone around it. These observations suggest an Ostwald ripening mechanism of the in meso crystal growth. Analysis of the microcrystal spatial distribution suggests that microcrystal nucleation occurs predominately at the LCP domain boundaries. |
| Databáze: | OpenAIRE |
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