EVALUATION OF WOUND HEALING AND ANTIBACTERIAL PROPERTIES OF METHANOLIC ROOT EXTRACT OF STEPHANIA DINKLAGEI (ENGL.) DIELS
| Autor: | Thoephilus Okafor Nnaji, Madubuike Umunna Anyanwu, S. O. Udegbunam, Nnabuike Prince Igbokwe, R. I. Udegbunam |
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| Rok vydání: | 2015 |
| Předmět: |
Minimum bactericidal concentration
Traditional medicine biology Granulation tissue Malondialdehyde medicine.disease_cause biology.organism_classification Microbiology chemistry.chemical_compound Minimum inhibitory concentration Hydroxyproline medicine.anatomical_structure Complementary and alternative medicine chemistry Staphylococcus aureus Drug Discovery medicine Stephania Wound healing |
| Zdroj: | African Journal of Traditional, Complementary and Alternative Medicines. 12:122-130 |
| ISSN: | 2505-0044 0189-6016 |
| DOI: | 10.21010/ajtcam.v12i6.12 |
| Popis: | Background: Stephania dinklagei Diels (Engl.) is used in folkloric medicine in Southeastern Nigeria for the treatment of wounds and some bacterial-associated infections. This study evaluated the wound healing and antibacterial potential of Stephania dinklagei to validate or invalidate its folkloric use. Materials and Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of methanolic extract of S. dinklagei root (MESDR) against Pseudomonas aeruginosa , Bacillus subtilis , Staphylococcus aureus , Escherichia coli and Klebsiella spp. was determined by macro broth dilution. The extract at 20% and 10% were dosed orally to rats at 300mg/kg body weight (bw) in incision and dead space wound healing model to determine wound tensile strength and granulation tissue weight, respectively. The same extract concentrations were applied topically in excision wound model to determine the rate of wound contraction and epithelialization. Activities of superoxide dismutase (SOD) and catalase (CAT), and the levels of total protein (TP), malondialdehyde (MAL), hydroxyproline (HYP) and hexosamine (HEX) in excision wound biopsies were determined at days 7 and 14 post-wounding (pw). In the excision wound model, the extract concentrations were compared with gentamicin sulphate. Results: The MIC of S. dinklagei extract against P. aeruginosa , S. aureus , B. subtilis , E. coli and Klebsiella spp. were 8mg/ml, 3 mg/ml, 5mg/ml, 6mg/ml and 7mg/ml, respectively, while the corresponding MBC were 10 mg/ml, 5 mg/ml, 7mg/ml, 8mg/ml, and 9 mg/ml, respectively. The 20% extract gave significantly ( P 0.05) at day 14 pw. SOD and CAT activities, and MDA and HEX level of all the groups did not vary significantly ( P >0.05) at day 14 pw. HYP level of the extract-treated groups significantly ( P |
| Databáze: | OpenAIRE |
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