A potential application for large diameter pellicular supports in the reversed phase study of ultra high molecular mass polystyrenes
| Autor: | Ian Maxwell Russell, P. E. Kavanagh, Ross Andrew Shalliker |
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| Rok vydání: | 1995 |
| Předmět: |
Chromatography
Molecular mass Elution Organic Chemistry Clinical Biochemistry Size-exclusion chromatography Analytical chemistry Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Polymer degradation chemistry Phase (matter) Polystyrene |
| Zdroj: | Chromatographia. 40:307-312 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02290362 |
| Popis: | Ultra high molecular mass (> 7 million dalton) polystyrenes are prone to degradation in size exclusion chromatography. In gradient elution reversed phase HPLC they do not elute visibly on small particle size porous supports. However, large diameter C18 pellicular particles were successfully employed for reversed phase study of ultra high molecular mass (15 million dalton) polystyrenes without polymer degradation during elution. Although retention for the lower molecular mass polystyrenes was lower than on small diameter porous particles, the medium high molecular mass polystyrenes (0.5–1 million dalton) showed similar retention. The addition of small diameter porous particles in small quantities, to the large diameter pellicular particles, increased the amount of retention of the low molecular mass polystyrenes without affecting the higher molecular mass polystyrenes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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