| Popis: |
All nuclear receptors reside in the nuclear compartment when transcriptionally active. However, their subcellular localization can vary when unliganded, functionally inactive, or dysregulated in diseased conditions. This property of differential localization and inducible nuclear translocation of nuclear receptors has proven to be useful tool in characterizing their transcription functions, discovery of novels drugs, ligands (agonists, antagonists, selective modulators of receptor action, endocrine disruptors, endobiotics, xenobiotics, etc.), partner-mediated interactions and coregulators. To efficiently study these ligand-modulated transcription factors, we describe a simple method for visual assessment and quantification of subcellular localization achieved by these receptors under varying physiological conditions. Contrary to some of the emerging high-end instrument-based assessments, the current method is simple, economical and highly reproducible, giving options to conduct similar studies in less sophisticated settings. It is expected that the current assessment approach will help investigators in their discovery of mechanisms of actions of these receptors in health and disease, and also in defining novel small molecule modulators to overcome physiological perturbations. |
