Splitting of Soil Humic Acid Fluorescence on Different Fluorophores

Autor:	Oleg Trubetskoj, Lubov Shaloiko, Olga Trubetskaya, Dmitrii V. Demin, Victor V. Marchenkov
Rok vydání:	2012
Předmět:	chemistry.chemical_classification Electrophoresis chemistry.chemical_compound Chromatography Fluorophore Membrane chemistry Analytical chemistry Urea Ultrafiltration Humic acid Fluorescence Polyacrylamide gel electrophoresis
Zdroj:	Functions of Natural Organic Matter in Changing Environment ISBN: 9789400756335
DOI:	10.1007/978-94-007-5634-2_9
Popis:	Polyacrylamide gel electrophoresis of chernozem soil humic acids (HA) followed by observation under UV light reveal new low molecular weight (MW) fluorescent fractions. Ultrafiltration of HA sample in 7 M urea on a membrane of low nominal MW retention (NMWR, 5 kDa) was repetitively used for separation of fluorescent and nonfluorescent species. Retentate R>5 kDa demonstrated very weak fluorescence under 270 nm excitation, while fluorescence intensity of ultrafiltrate UF
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4d0c11f5489d0d1cdd773f4f59cdcee0 https://doi.org/10.1007/978-94-007-5634-2_9 Zobrazit plný text záznamu