Isolation and identification of endophytic fungi from two medicinally important orchids, Satyrium nepalense D. Don and Herminium lanceum (Thunb. ex sw.)Vuijk
| Autor: | Akanksha Priya, Saloni Malik, Shashi B. Babbar, Deepak Singh, Rupam Kapoor |
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| Rok vydání: | 2017 |
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| Zdroj: | Medicinal Plants - International Journal of Phytomedicines and Related Industries. 9:95 |
| ISSN: | 0975-6892 0975-4261 |
| DOI: | 10.5958/0975-6892.2017.00014.4 |
| Popis: | Endophytic fungi isolated from medicinal plants can be good sources of medicine. Satyrium nepalense and Herminium lanceum are two medicinally important orchids. Decoction of stems, roots and tubers of Satyrium nepalense, locally known as ‘Salam mishri’, is prescribed in various ailments and also as dietary supplement since ancient times. H. lanceum, locally known as ‘Jalya’, is used for the treatment of urinary problems, diabetes, fever and haemorrhagia. A total of 12 fungal species, eight and four, respectively, were isolated from the root cultures of S. nepalense and H. lanceum. For the identification of these isolates, nuclear internal transcribed spacer (ITS) from each of these was amplified and sequenced. The amplification and sequencing of ITS was successful from 11 of the 12 fungi. All the amplicons yielded barcode quality sequences, which have been submitted to the GenBank, NCBI. The fungi were identified by BLAST method on NCBI as well as BOLD. Five fungal species, Ilyonectria estremocensis, Cryptosporiopsis ericae, Thanatephorus cucumeris, Phialophora mustea, Aspergillus species from S. nepalense, and two from H. lanceum, Fusarium falciforme and Aspergillus flavus, were identified. Most of these fungi have been shown to be antibacterial and/or anti-cancerous, and a few are the known to be sources of bioactive molecules. |
| Databáze: | OpenAIRE |
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