| Autor: |
Straumal, B.B., Gornakova, A.S., Kiselevskiy, M.V., Anisimova, N.Yu., Nekrasov, A.N., Strug, R., Rabkin, E. |
| Jazyk: |
ruština |
| Rok vydání: |
2022 |
| Předmět: |
|
| DOI: |
10.26201/issp.2022/fppk.145 |
| Popis: |
The wetting behavior of surfaces strongly depends on their roughness. In this work we investigated the effect of surface roughness of Ti - 6 wt.%Al - 4 wt.% V alloy on the adhe-sion of HEK293 human cells. The array of linear scratches has been produced on the surface of cold-wrought Ti - 6 wt.%Al - 4 wt.% V alloy discs with the aid of various abrasive SiC papers with grit sizes of 220, 400, 600, 800, 1000, 1200, 2000 and 4000 (number of grains per cm2) and different diamond pastes with grain sizes 6, 3 and 1 μm. These SiC papers grades correspond to the SiC grain size of 68-7 μm. The resulted surface topography of the samples was examined by the conventional and confocal light microscopy and high-resolution scan-ning electron microscopy. Afterwards, the adhesion of the HEK293 human cells to the sam-ples of different roughness has been measured by fluorescence miscroscopy. The size and morphology of adhered cells were measured by the light miscroscopy. The fraction of adhered cells behaves non-monotonously with grain size. It is about 0.35 for grain size of 1 μm, it in-creases with increasing grain size up to 0.95 for grain size of 7 μm and then slowly decreases down to 0.55 at abrasive grain size of 68 μm. The position of maximum is comparable with the dimension of HEK293 cells (~14 μm). Thus, the variation of surface roughness opens the way to control and tailor the fraction of adhered cells, depending on demand of medical tech-niques. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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