High-Sensitivity Dry Rehydratable Film Method for Enumeration of Coliforms in Dairy Products: Collaborative Study

Autor:	McAllister Js, D'onorio A, Gambrel-Lenarz S, Gangar, Curiale Ms
Rok vydání:	1997
Předmět:	Pharmacology food.ingredient food and beverages Solid medium food.food Analytical Chemistry Agar plate food Multicenter study Ice cream Chocolate milk Enumeration Environmental Chemistry Agar Food science Agronomy and Crop Science Food Science Mathematics
Zdroj:	Journal of AOAC INTERNATIONAL. 80:505-516
ISSN:	1944-7922 1060-3271
DOI:	10.1093/jaoac/80.3.505
Popis:	A dry-film coliform count plate that is inoculated with 5 mL sample was compared with the Violet Red Bile Agar plate method in a collaborative study by 18 laboratories. Products analyzed were 2% milk, chocolate milk, cream, vanilla ice cream, cottage cheese, and cheese. Collaborators tested blind duplicate uninoculated samples and samples inoculated at low, medium, and high level. Significantly (P< 0.05) higher numbers of coliforms were recovered by the dry-film method from 2% milk samples at the 3 inoculum levels, the chocolate milk at the low- and high-inoculum levels, and the cream at the high-inoculum level. Significantly higher counts were obtained by the agar method for cottage cheese samples at the low-inoculum level. The repeatability standard deviation for the dry-film method was significantly higher for the high-inoculum level chocolate milk sample and the medium-inoculum level cottage cheese. The same statistic was significantly higher for the agar method at all 3 inoculum levels in the 2% milk and the medium-inoculum level cream. The high-sensitivity dry rehydratable film method for enumeration of coliforms in dairy products has been adopted first action by AOAC INTERNATIONAL.
Databáze:	OpenAIRE
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