Solid-phase enzyme immunoassay for hepatitis B surface antigen
| Autor: | H E Bond, G J Knight, R Wei, D H Zimmerman |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
chemistry.chemical_classification
Chromatography medicine.diagnostic_test biology Chemistry Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Hepatitis b surface antigen Molecular biology Horseradish peroxidase Enzyme Immunoassay Immunoenzyme techniques Phase (matter) medicine biology.protein Antibody |
| Zdroj: | Clinical Chemistry. 23:813-815 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/23.5.813 |
| Popis: | A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specificity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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