Cyanobacteria as test organisms and biosorbents
| Autor: | Anna I. Fokina, L. V. Kondakova, E. I. Lyalina, E. A. Gornostaeva, L. I. Domracheva, S. Yu. Ogorodnikova, T. Ya. Ashikhmina |
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| Rok vydání: | 2017 |
| Předmět: |
Pheophytin
Cyanobacteria 0209 industrial biotechnology biology Biosorption Soil Science Dehydrogenase 04 agricultural and veterinary sciences 02 engineering and technology biology.organism_classification Nostoc commune chemistry.chemical_compound 020901 industrial engineering & automation chemistry Catalase Chlorophyll Botany 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries Bioassay Earth-Surface Processes Nuclear chemistry |
| Zdroj: | Eurasian Soil Science. 50:70-77 |
| ISSN: | 1556-195X 1064-2293 |
| DOI: | 10.1134/s106422931611003x |
| Popis: | Bioassay and biosorption potentials of different groups of cyanobacteria (CB)—Nostoc linckia (Roth.) Born. et Flah. No. 271, natural biofilms dominated by CB of Phormidium genus, and biofilms dominated by Nostoc commune (Vauch. Elenk)—were estimated. The physiological-biochemical response of CB to the influence of copper sulfate (II) (catalase activity with a gasometric method and dehydrogenase activity, lipid peroxidation, and chlorophyll ɑ and pheophytin contents with a spectrophotometric method) was studied; metal bioaccumulation was determined with a stripping voltammetry method. It was found that the communities dominated by Phormidium genus (CB biomass 0.2 g/dm3) removed copper compounds from the solutions with Cu2+ ion concentration of 20 mg/dm3 almost completely (by 99%); communities dominated by CB N. commune, by 87%; and pure culture of N. linckia, by 50%. Dehydrogenase and catalase activities and the intensity of bioluminescence proved to be sensitive indicators of the response of CB to Cu2+ ions. The impact of Cu2+ ions (20 mg/dm3) on a biofilm dominated by CB of Phormidium genus resulted in the fivefold decrease of catalase activity during 24 h; dehydrogenase activity decreased by nearly 357 times. The bioluminescence intensity during 24 h decreased by 1.3–100 times under the impact of Cu2+ (2 mg/dm3) and by 8.6–200 times in variants with a higher concentration of Cu2+ (20 mg/dm3). This regularity can be used as a test function in bioassay. |
| Databáze: | OpenAIRE |
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