Simultaneous determination of praziquantel, pyrantel embonate, febantel and its active metabolites, oxfendazole and fenbendazole, in dog plasma by liquid chromatography/mass spectrometry
| Autor: | K Ary, Péter Székely-Körmöczy, Kálmán Róna, Eva Keller, Péter Laczay, Péter Sótonyi, Zoltán Sára, Gabriella Klausz |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
Oxfendazole Chromatography Chemistry Clinical Biochemistry General Medicine Bioequivalence Biochemistry Analytical Chemistry Liquid chromatography–mass spectrometry Pyrantel Drug Discovery medicine Fenbendazole Solid phase extraction Anthelmintic Molecular Biology Active metabolite medicine.drug |
| Zdroj: | Biomedical Chromatography. 29:1859-1865 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.3507 |
| Popis: | A liquid chromatography–electrospray–mass spectrometry method (LC/MS) has been developed and validated for determination of praziquantel (PZQ), pyrantel (PYR), febantel (FBT), and the active metabolites fenbendazole (FEN) and oxfendazole (OXF), in dog plasma, using mebendazole as internal standard (IS). The method consists of solid-phase extractions on Strata-X polymeric cartridges. Chromatographic separation was carried out on a Phenomenex Gemini C6-Phenyl column using binary gradient elution containing methanol and 50 mm ammonium–formate (pH 3). The method was linear (r2 ≥ 0.990) over concentration ranges of 3–250 ng/mL for PYR andFEB, 5–250 ng/mL for OXF and FEN, and 24–1000 ng/mL for PZQ. The mean precisions were 1.3–10.6% (within-run) and 2.5–9.1% (between-run), and mean accuracies were 90.7–109.4% (within-run) and 91.6–108.2% (between-run). The relative standard deviations (RSD) were |
| Databáze: | OpenAIRE |
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