The Adjuvant Effect of Sophy ¥-Glucan to the Antibody Response in Poultry Immunized by the Avian Influenza A H5N1 and H5N2 Vaccines

Autor:	Yasunori Ikeue, Haruka Nomura, Viet Cuong Pham, Tran Binh Le, Kim Xuyen Thi Le, Thanh Hoa Le, Takeshi Agatsuma, Mitsuru Nagataki, Dong Van Quyen, Yoshiya Watanabe, Thanh Huong Thi Doan
Rok vydání:	2011
Předmět:	Hemagglutination assay biology medicine.medical_treatment Immunogenicity General Medicine medicine.disease_cause Applied Microbiology and Biotechnology Virology Influenza A virus subtype H5N1 Microbiology Antigen Immunity medicine biology.protein Antibody Seroconversion Adjuvant Biotechnology
Zdroj:	Journal of Microbiology and Biotechnology. 21:405-411
ISSN:	1738-8872 1017-7825
DOI:	10.4014/jmb.1011.11024
Popis:	Avian influenza virus vaccines produced in oil-emulsified inactivated form with antigen content of at least 160 hemagglutinin units (HAU) induced immunity in birds. However, in addition to enhancing the effect of the adjuvant(s), other additional supplemented biological compounds included in inactivated vaccines could produce higher levels of antibody. We examined in chickens, Vietnamese ducks, and muscovy ducks the adjuvant effect of Sophy β-glucan (SBG), a β-1,3-1,6 glucan produced by the black yeast Aureobasidium pollulans strain AF0-202, when administered with an avian influenza H5 subtype vaccine. In Experiment 1, 40 chickens (ISA Brown hybrid), allocated to four groups of ten each, were immunized with Oil-H5N1(VN), Oil-H5N1(CN), Oil-H5N2(CN), and saline (control group), respectively. In Experiment 2, chickens (ISA Brown hybrid), muscovy ducks (French hybrid), and Vietnamese ducks (indigenous Vietnamese) were used to further assess the effect of SBG on immunogenicity of the Oil-H5N1(VN) Vietnamese vaccine. ELISA and hemagglutination inhibition (HI) assays were used to assess the antibody response. The H5 subtype vaccines initiated significantly higher immune responses in the animals dosed with SBG, with 1.0-1.5 log2 higher HI titers and 10-20% ELISA seroconversion, compared with those not dosed with β-glucan. Notably, some of the animals dosed with SBG induced HI titers higher than 9.0 log2 following boosting immunization. Taken together, our serial studies indicated that SBG is a potential effector, such as enhancing the immune response to the H5 vaccines tested.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4cca31b2f51ec9dfc68167fac55f17a6 https://doi.org/10.4014/jmb.1011.11024 Zobrazit plný text záznamu