Cytological Techniques to Study Cytomixis in Plant Male Meiosis
| Autor: | Sergey Mursalimov, Elena V. Deineko, Yuriy V. Sidorchuk |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Secale biology medicine.diagnostic_test Nicotiana tabacum fungi Callose Cytomixis food and beverages Meiocyte biology.organism_classification Plant cell 01 natural sciences humanities Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Meiosis medicine 010606 plant biology & botany Fluorescence in situ hybridization |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493998173 |
| Popis: | In this chapter we describe cytological techniques to study cytomixis, a process of nuclear migration between plant cells, in squashed plant male meiocytes of Nicotiana tabacum and Secale cereale. To perform immunostaining or fluorescence in situ hybridization (FISH) on meiotic cells involved in cytomixis common protocols are modified. During preparation of specimens for subsequent cytological analysis, it is necessary not only to make DNA and proteins accessible to DNA probes and antibodies, but also to preserve cell cytoplasm. There are also some important modifications in the protocols applied for meiocytes of different plant species. Here we describe protocols for immunostaining and FISH in rigid tobacco male meiocytes with dense cytoplasm and thick callose wall, that tolerate hard squashing, and in soft rye male meiocytes, that are easily damaged upon squashing, both to study cytomixis. |
| Databáze: | OpenAIRE |
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