Characterization of the Tumorlike (T) Antigen Induced by Type 12 Adenovirus

Autor:	Harold S. Ginsberg, Z. Gilead
Rok vydání:	1968
Předmět:	Mouth neoplasm Immunology Analytical Centrifugation Biology Trypsin Microbiology Molecular biology Sedimentation coefficient Isoelectric point Antigen Virology Insect Science medicine Centrifugation Ultracentrifuge medicine.drug
Zdroj:	Journal of Virology. 2:15-20
ISSN:	1098-5514 0022-538X
DOI:	10.1128/jvi.2.1.15-20.1968
Popis:	T antigen, purified from KB cells infected with type 12 adenovirus, was characterized by chemical and physical methods. The immunologically active molecule had an ultraviolet-absorption spectrum characteristic of a protein, and was partially inactivated (i.e., approximately 85%) by trypsin (final concentration of 150 μg/ml). Centrifugation analyses indicated that the purified antigen was a single molecular species with an average sedimentation coefficient of 2.40 S (measured by zonal centrifugation in sucrose gradients) and an S 20,w of 2.58 determined by analytical centrifugation. The antigen was inactivated at temperatures above 40 C, it was stable between p H 5.7 and 8.8, and it had an isoelectric point of approximately 5.0, as determined by electrophoresis on cellulose polyacetate membranes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4cc559246904c2cdf9b265d2dda8d0a3 https://doi.org/10.1128/jvi.2.1.15-20.1968 Zobrazit plný text záznamu